In-situ visualization of sound-induced otolith motion using hard X-ray phase contrast imaging

Author(s)
Tanja Schulz-Mirbach, Margie Olbinado, Alexander Rack, Alberto Mittone, Alberto Bravin, Roland R. Melzer, Friedrich Ladich, Martin Heß
Abstract

Regarding the basics of ear structure-function relationships in fish, the actual motion of the solid otolith relative to the underlying sensory epithelium has rarely been investigated. Otolith motion has been characterized based on a few experimental studies and on approaches using mathematical modeling, which have yielded partially conflicting results. Those studies either predicted a simple back-and-forth motion of the otolith or a shape-dependent, more complex motion. Our study was designed to develop and test a new set-up to generate experimental data on fish otolith motion in-situ. Investigating the basic parameters of otolith motion requires an approach with high spatial and temporal resolution. We therefore used hard X-ray phase contrast imaging (XPCI). We compared two anatomically well-studied cichlid species, Steatocranus tinanti and Etroplus maculatus, which, among other features, differ in the 3D shape of their otoliths. In a water-filled tank, we presented a pure tone of 200 Hz to 1) isolated otoliths embedded in agarose serving as a simple model or 2) to a fish (otoliths in-situ). Our new set-up successfully visualized the motion of otoliths in-situ and therefore paves the way for future studies evaluating the principles of otolith motion.

Organisation(s)
External organisation(s)
Ludwig-Maximilians-Universität München, Zoologische Staatssammlung München (ZSM), European Synchrotron Radiation Facility ESRF
Journal
Scientific Reports
Volume
8
ISSN
2045-2322
DOI
https://doi.org/10.1038/s41598-018-21367-0
Publication date
12-2018
Peer reviewed
Yes
Austrian Fields of Science 2012
106051 Behavioural biology
ASJC Scopus subject areas
General
Portal url
https://ucris.univie.ac.at/portal/en/publications/insitu-visualization-of-soundinduced-otolith-motion-using-hard-xray-phase-contrast-imaging(5a4adfb3-a45d-444e-935b-ea7850a67c49).html